A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and designated as TaHIR2.The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) illumivein were 31.05 kD and 5.18, respectively.
Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein.Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2.Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells.Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage.
These results suggest that TaHIR2 may play an the beast sullivan supply active role in wheat defense against stripe rust.